Lyme Central Nervous System Infection IgG with Antibody Index Reflex, Serum and Spinal Fluid, Mayo, RefLab
This test begins with IgG screening of the spinal fluid (CSF) specimen. If the screen is negative, no additional testing will be performed.
If the screen is positive, the paired CSF and serum specimens will be used to establish the antibody index. In order to establish the antibody index, the paired serum and CSF samples (collected within 24 hours of each other) are tested on the same run using quantitative assays to determine levels for the following analytes:
1. Anti-Borrelia species IgG levels in CSF and serum
2. Total IgG in CSF and serum
3. Albumin in CSF and serum
Reference Lab
(maximum) of each other.
CSF: Sterile vial
Serum: SST (Gold Top Serum Separator Tube)
Serum:
RST -(Orange Top Serum Separator Tube)
Plain Red
Red/Black Speckle Top Serum Tube
Serum: 2.4 mL
Serum: 2.4 mL
CSF
1. A CSF sample of 1.2 mL needs to be collected within 24 hours of the serum specimen, preferably at the same time.
2. Label vial as spinal fluid or CSF.
3. CSF aliquot should be from the second, third, or fourth CSF vial collected during the lumbar puncture.
- Do not submit CSF from the first vial due to the possibility of blood contamination, which will cause specimen rejection.
4. Band serum and CSF specimens together.
SERUM
1. A serum sample of 1.2 mL needs to be collected within 24 hours of the spinal fluid specimen, preferably at the same time.
2. Centrifuge and aliquot serum into a plastic vial.
3. Label as serum.
4. Band serum and CSF specimens together.