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Hemoglobin, Free, Plasma

Alternate Names
005595 LC
SoftLab Order Code
HGBPL
Epic Order Name
HEMOGLOBIN, FREE, PLASMA, REF LAB LC 005595
Epic Order ID
LAB92
Laboratory

Reference Lab

Additional Test Information

Results of this test are labeled for research purposes only by the assay's manufacturer. The performance characteristics of this assay have not been established by the manufacturer. The result should not be used for treatment or for diagnostic purposes without confirmation of the diagnosis by another medically established diagnostic product or procedure. The performance characteristics were determined by LabCorp.

Sample Type
Plasma
Collection Container

Green No Gel (Sodium Heparin)

green top

Alternative Collection Container

Green No Gel (Lithium Heparin)

Collecting Volume
2.0 mL
Minimum Collecting Volume
0.5 mL
Storage or Stability
Refrigerated
Causes for Rejection
Traumatic venipuncture causing hemolysis; very icteric or lipemic specimen; specimen not heparinized plasma
Instructions

Recommended procedure for collecting sample without inducing hemolysis

  • An 18-gauge needle with attached infusion tubing is used.
  • Place the tourniquet lightly around the upper arm. Puncture an antecubital vein with as little trauma as possible.
  • Release the tourniquet and clamp the tubing off as soon as blood return is seen.
  • First collect 3 mL blood in a red-top tube with the rubber top off.
  • Then, collect 5 mL in an anticoagulated (heparin) tube with the top off.
  • Clamp the tubing, withdraw the needle, and apply a pressure bandage to the site until residual bleeding is stopped.
  • Recap the anticoagulant tube and gently mix three to five times.
  • This specimen is used for the plasma hemoglobin determination.
  • Centrifuge and separate plasma from red cells immediately following collection and transfer plasma to a transfer tube. 
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