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Name of Test
Pyruvic Acid, Blood
Alternate Names
004788 LC, Pyruvate
SoftLab Order Code
PYRUV
Epic Order Name
PYRUVIC ACID, BLOOD, REF LAB LC 004788
Epic Order ID
LAB744
Laboratory
RefLab
Additional Test Information
Draw blood into chilled gray-top tube. Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). Note: Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained. Shake mixture vigorously for about 30 seconds. Refrigerate for five minutes to ensure complete protein precipitation. Centrifuge 5 to 10 minutes at approximately 1500xg. Immediately transfer the clear supernatant to a transport tube. Label the transport tube as "supernatant" and send to the laboratory. Note: A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.
Sample Type
Perchloric acid supernatant from oxalated whole blood  
Collection Container
Gray Sodium Flouride on ICE
Collecting Volume
3.0 ml
Minimum Collecting Volume
2.0 ml
Storage or Stability
Refrigerated
Causes For Rejection
Whole blood received; gray-top tube NOT prepared with perchloric acid  
Patient Preparation
Patient should be in a fasting and resting state (should not exercise).  
 
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